1SZS
The structure of gamma-aminobutyrate aminotransferase mutant: I50Q
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | SIEMENS |
| Temperature [K] | 100 |
| Detector technology | AREA DETECTOR |
| Collection date | 2000-10-18 |
| Detector | SIEMENS HI-STAR |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 108.100, 108.100, 301.700 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.100 |
| R-factor | 0.17 |
| Rwork | 0.169 |
| R-free | 0.20600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.530 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 115032 | |
| Completeness [%] | 95.8 | 95.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 293 | HEPES, ammonium sulfate, PLP, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
