1SYI

X-RAY STRUCTURE OF THE Y702F MUTANT OF THE GLUR2 LIGAND-BINDING CORE (S1S2J) IN COMPLEX WITH (S)-CPW399 AT 2.1 A RESOLUTION.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	110
Detector technology	CCD
Collection date	2002-11-28
Detector	MARRESEARCH
Wavelength(s)	0.8111
Spacegroup name	P 41
Unit cell lengths	47.719, 47.719, 237.401
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.420 - 2.100
Rwork	0.206
R-free	0.25400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1syh
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.086	0.347
Number of reflections	28467
<I/σ(I)>	10.3	3.3
Completeness [%]	92.3	90.2
Redundancy	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	280	PEG8000, CACODYLATE, (NH4)2SO4, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 280K