1SX8
EcoRV bound to cognate DNA and Mn2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.800, 49.100, 63.700 |
| Unit cell angles | 96.90, 108.90, 107.10 |
Refinement procedure
| Resolution | 20.000 - 2.150 |
| Rwork | 0.213 |
| R-free | 0.25400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.910 |
| Data reduction software | MOSFLM |
| Phasing software | X-PLOR |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.180 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.089 | 0.307 |
| Number of reflections | 23851 | |
| <I/σ(I)> | 3.8 | 2 |
| Completeness [%] | 0.9 | 89.7 |
| Redundancy | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | PEG 4000, 0.1M HEPES, 0.15M NaCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 100K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 4000 | ||
| 2 | 1 | 2 | HEPES | ||
| 3 | 1 | 3 | NaCl |
