1STH

TWO DISTINCTLY DIFFERENT METAL BINDING MODES ARE SEEN IN X-RAY CRYSTAL STRUCTURES OF STAPHYLOCOCCAL NUCLEASE-COBALT(II)-NUCLEOTIDE COMPLEXES

Experimental procedure

Spacegroup name	P 41
Unit cell lengths	47.480, 47.480, 63.210
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 1.850
R-factor	0.174
Rwork	0.174
RMSD bond length	0.014
RMSD bond angle	25.100 *
Phasing software	X-PLOR (3.0)
Refinement software	X-PLOR (3.0)

Data quality characteristics

	Overall
Low resolution limit [Å]	6.000 *
High resolution limit [Å]	1.850 *
Rmerge	0.048 *
Total number of observations	87667 *
Number of reflections	11570 *
Completeness [%]	99.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	potassium phosphate	10.5 (mM)
3	1	drop	MPD	17 (%)
4	1	drop	pdTp	2 (mg/ml)
5	1	drop		10 (mM)
6	1	drop	potassium citrate	20 (mM)
7	1	reservoir	MPD	21-23 (%(w/w))
8	1	reservoir	potassium phosphate	10.5 (mM)