1SSX
0.83A resolution crystal structure of alpha-lytic protease at pH 8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-1 |
Synchrotron site | SSRL |
Beamline | BL9-1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2000-01-07 |
Detector | MARRESEARCH |
Wavelength(s) | 0.785 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 65.910, 65.910, 79.697 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 10.000 - 0.830 |
R-factor | 0.087 |
Rwork | 0.086 |
R-free | 0.09910 |
Structure solution method | REFINEMENT OF 1TAL.PDB |
Starting model (for MR) | 1tal |
RMSD bond length | 0.025 |
RMSD bond angle | 0.046 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 10.000 | 0.840 |
High resolution limit [Å] | 0.830 | 0.830 |
Rmerge | 0.059 | 0.407 |
Number of reflections | 187431 | |
<I/σ(I)> | 28.5 | 3 |
Completeness [%] | 99.6 | 98.8 |
Redundancy | 4.3 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 1.3M lithium sulfate, 0.02M Tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |