1SR9
Crystal Structure of LeuA from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | MAD |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-07-08 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.250, 154.730, 68.820 |
| Unit cell angles | 90.00, 98.05, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.17 |
| Rwork | 0.169 |
| R-free | 0.19700 |
| Structure solution method | MAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.470 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 74305 | |
| Completeness [%] | 98.4 | 94.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.25 | 291 | sodium citrate, MPEG2000, pH 6.25, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
