1SO0
Crystal structure of human galactose mutarotase complexed with galactose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 275 |
Detector technology | AREA DETECTOR |
Collection date | 2004-02-10 |
Detector | SIEMENS HI-STAR |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 |
Unit cell lengths | 61.000, 68.700, 98.900 |
Unit cell angles | 107.70, 98.40, 102.70 |
Refinement procedure
Resolution | 30.000 - 2.300 |
R-factor | 0.169 |
Rwork | 0.167 |
R-free | 0.19800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1snz |
RMSD bond length | 0.013 |
RMSD bond angle | 2.400 |
Data reduction software | SAINT |
Data scaling software | SAINT |
Phasing software | AMoRE |
Refinement software | TNT |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.400 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 61156 | |
<I/σ(I)> | 10.8 | 2.8 |
Completeness [%] | 94.7 | 77.5 |
Redundancy | 2.1 | 1.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | batch | 6 | 275 | PEG 8000, sodium chloride, lithium chloride, galactose, MES, pH 6.0, batch, temperature 275K |