1SL0
Ternary 3' complex of T7 DNA polymerase with a DNA primer/template containing a disordered cis-syn thymine dimer on the template and an incoming nucleotide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-07-11 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.266, 105.466, 213.931 |
| Unit cell angles | 90.00, 91.57, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.200 |
| R-factor | 0.282 |
| Rwork | 0.282 |
| R-free | 0.35100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.310 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Number of reflections | 39978 | |
| <I/σ(I)> | 35.2 | 5.67 |
| Completeness [%] | 99.4 | 96.1 |
| Redundancy | 34.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | pH 7.5, VAPOR DIFFUSION, HANGING DROP |
