1SHY
The Crystal Structure of HGF beta-chain in Complex with the Sema Domain of the Met Receptor.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-09-07 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.928 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 137.052, 186.352, 66.659 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.220 |
| R-factor | 0.21206 |
| Rwork | 0.209 |
| R-free | 0.26978 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | HGF beta chain |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.537 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.310 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Number of reflections | 28312 | |
| Completeness [%] | 99.4 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 292 | PEG, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
