1SHW

EphB2 / EphrinA5 Complex Structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X9A
Synchrotron site	NSLS
Beamline	X9A
Temperature [K]	100
Detector technology	CCD
Collection date	2003-12-01
Detector	MARRESEARCH
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	37.890, 88.970, 122.810
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.200
Rwork	0.258
R-free	0.30500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	EphB2/ EphrinB2
RMSD bond length	0.006
RMSD bond angle	1.370
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.090
High resolution limit [Å]	2.000	2.000
Rmerge	0.051
Number of reflections	21147
<I/σ(I)>	15.8
Completeness [%]	98.6	99.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	278	PEG 3350, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 278K