1SEH
Crystal structure of E. coli dUTPase complexed with the product dUMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-05 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8040 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 75.257, 75.257, 100.532 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.470 |
| R-factor | 0.15895 |
| Rwork | 0.158 |
| R-free | 0.18384 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1euw |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.379 |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.600 |
| High resolution limit [Å] | 1.470 | 1.470 |
| Number of reflections | 29251 | |
| <I/σ(I)> | 27.6 | 4.86 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 31.8 | 20.09 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 293 | PEG 3350, SODIUM ACETATE, TRIS, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
