1SA1

Tubulin-podophyllotoxin: stathmin-like domain complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2000-06-10
Detector	ADSC
Wavelength(s)	0.933
Spacegroup name	P 65
Unit cell lengths	328.061, 328.061, 54.301
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 4.200
R-factor	0.20643
Rwork	0.204
R-free	0.25935
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1sa0
RMSD bond length	0.019
RMSD bond angle	2.091
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	4.300
High resolution limit [Å]	4.200	4.200
Rmerge	0.060	0.482
Number of reflections	24624
<I/σ(I)>	18	2
Completeness [%]	98.3	90.2
Redundancy	3.26	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	Gigant, B., (2000) Cell, 102, 809. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	reservoir	PEG