1S9K
Crystal Structure of Human NFAT1 and Fos-Jun on the IL-2 ARRE1 Site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1996-09-16 |
| Detector | BRANDEIS - B4 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.769, 86.260, 84.044 |
| Unit cell angles | 90.00, 111.24, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.100 |
| R-factor | 0.2423 |
| Rwork | 0.242 |
| R-free | 0.27460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.200 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Number of reflections | 16172 | |
| Completeness [%] | 96.9 | 71.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 300-400 mM Ammonium Acetate Salt, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Ammonium Acetate Salt | ||
| 2 | 1 | 1 | H2O | ||
| 3 | 1 | 2 | Ammonium Acetate Salt | ||
| 4 | 1 | 2 | H2O |
