1S4Y

Crystal structure of the activin/actrIIb extracellular domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2003-05-21
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0332
Spacegroup name	P 21 21 2
Unit cell lengths	86.290, 120.627, 43.550
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	70.710 - 2.300
R-factor	0.21021
Rwork	0.206
R-free	0.28615
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.031
RMSD bond angle	2.385
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	EPMR
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	19028
Completeness [%]		94.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.7	277	PEG 3350, magnesium chloride, bis tris, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 277K