1S1L
Influence of Groove Interactions on the Formation of DNA Holliday Junctions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 103 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-11-08 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.542 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 64.923, 24.869, 37.095 |
| Unit cell angles | 90.00, 109.22, 90.00 |
Refinement procedure
| Resolution | 18.200 - 2.200 |
| R-factor | 0.236 |
| Rwork | 0.236 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | ndb id UD0019 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.800 |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.200 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.050 | 0.291 |
| Number of reflections | 3492 | |
| <I/σ(I)> | 2.4 | |
| Completeness [%] | 93.4 | 61.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 25mM Na Cacodylate, .5mM DNA, 180mM CaCl2, 2.4mM spermine against 20% resevoir MPD, pH 7.0, VAPOR DIFFUSION, SITTING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Na Cacodylate | ||
| 2 | 1 | 1 | .5mM DNA | ||
| 3 | 1 | 1 | CaCl2 | ||
| 4 | 1 | 1 | spermine | ||
| 5 | 1 | 2 | MPD | ||
| 6 | 1 | 2 | Na Cacodylate |
