1S0V
Structural basis for substrate selection by T7 RNA polymerase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 |
| Synchrotron site | SPring-8 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 81.133, 87.703, 206.531 |
| Unit cell angles | 91.93, 91.02, 110.66 |
Refinement procedure
| Resolution | 40.000 - 3.200 |
| R-factor | 0.255 |
| Rwork | 0.255 |
| R-free | 0.30700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1h83 |
| RMSD bond length | 0.022 * |
| RMSD bond angle | 2.100 * |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.084 * | 0.461 * |
| Total number of observations | 229915 * | |
| Number of reflections | 94902 | |
| Completeness [%] | 89.4 | 83.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 293 * | Temiakov, D., (2003) Acta Crystallogr.,Sect.D, 59, 185. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | PEG8000 | 10 (%) | |
| 3 | 1 | reservoir | glycerol | 10 (%) | |
| 4 | 1 | reservoir | beta-mercaptoethanol | 5 (mM) | |
| 5 | 1 | reservoir | Tris | 100 (mM) | pH8.1 |
