1RXD
Crystal structure of human protein tyrosine phosphatase 4A1
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2003-06-19 |
Detector | MARRESEARCH |
Wavelength(s) | 0.97911, 0.97934, 0.97166 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 71.291, 105.568, 179.355 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 - 1.900 |
R-factor | 0.226 |
Rwork | 0.223 |
R-free | 0.24200 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.590 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.132 | 0.575 |
Number of reflections | 48954 | |
<I/σ(I)> | 14.6 | 3.2 |
Completeness [%] | 91.3 | 72.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | PEG 3350, HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |