1RXC
E. COLI uridine phosphorylase: 5-fluorouracil ribose-1-phosphate complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200H |
| Temperature [K] | 115 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-08-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 90.188, 191.701, 91.909 |
| Unit cell angles | 90.00, 118.50, 90.00 |
Refinement procedure
| Resolution | 16.930 - 2.350 |
| R-factor | 0.15267 |
| Rwork | 0.150 |
| R-free | 0.20250 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.214 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 16.930 | 2.470 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.109 | 0.419 |
| Number of reflections | 105171 | |
| <I/σ(I)> | 6.2 | 1.8 |
| Completeness [%] | 92.7 | 84.9 |
| Redundancy | 6.5 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH METHOD UNDER OIL | 7.5 | 289 | TRIS HCL, PEG4000, POTASSIUM ACETATE, 5-FLUOROURACIL, RIBOSE-1-PHOSPHATE, pH 7.50, BATCH METHOD UNDER OIL, temperature 289K |
