1RX7

STRUCTURE OF DIHYDROFOLATE REDUCTASE COMPLEXED WITH FOLATE

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	293
Detector technology	AREA DETECTOR
Collection date	1995-06
Detector	XUONG-HAMLIN MULTIWIRE
Spacegroup name	P 21 21 21
Unit cell lengths	34.990, 45.308, 101.138
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.300
R-factor	0.181 *
Rwork	0.181
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ra2
RMSD bond length	0.022
RMSD bond angle	2.980
Data reduction software	UCSD
Data scaling software	UCSD
Phasing software	MERLOT
Refinement software	TNT (5D)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.480
High resolution limit [Å]	2.300	2.300
Rmerge		0.175 *
Total number of observations	13988 *
Number of reflections	6569
<I/σ(I)>	8.3	2.1
Completeness [%]	87.0	81
Redundancy	2.1	1.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	macro seeding *	6.5		pH 6.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	cacodylate	20 (mM)
2	1	reservoir	PEG6000	20 (%(w/v))
3	1	reservoir		300 (mM)
4	1	reservoir	cacodylate	20 (mM)