1RWE
Enhancing the activity of insulin at receptor edge: crystal structure and photo-cross-linking of A8 analogues
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-D |
| Synchrotron site | APS |
| Beamline | 14-BM-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-08-19 |
| Detector | ADSC |
| Spacegroup name | H 3 |
| Unit cell lengths | 79.341, 79.341, 34.708 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.180 - 1.800 |
| R-factor | 0.189 |
| Rwork | 0.189 |
| R-free | 0.24500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1TRZ AND 1MPJ |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.180 | 1.910 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.048 | 0.224 |
| Number of reflections | 7426 | |
| <I/σ(I)> | 23.52 | 9.2 |
| Completeness [%] | 98.4 | 95.4 |
| Redundancy | 4.3 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.8 | 298 | Tris, sodium citrate, acetone, phenol, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 6.80 |
