1RT6
HIV-1 REVERSE TRANSCRIPTASE COMPLEXED WITH UC38
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX7.2 |
| Synchrotron site | SRS |
| Beamline | PX7.2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-09 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 137.000, 109.300, 71.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.800 |
| R-factor | 0.234 * |
| Rwork | 0.236 |
| R-free | 0.33500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | RT-MKC-442 COMPLEX (PDB ENTRY 1RT1) |
| RMSD bond length | 0.008 |
| RMSD bond angle | 24.100 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.069 | 0.357 |
| Total number of observations | 84517 * | |
| Number of reflections | 26073 | 2446 * |
| <I/σ(I)> | 13.9 | 1.91 |
| Completeness [%] | 95.7 | 91.3 |
| Redundancy | 3.24 | 2.65 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 5 | 4 * | pH 5.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | citric acid | 24.25 (mM) | |
| 2 | 1 | 1 | sodium phosphate | 51.5 (mM) | |
| 3 | 1 | 1 | PEG3400 | 6-10 (%(w/v)) |
