1RRG

NON-MYRISTOYLATED RAT ADP-RIBOSYLATION FACTOR-1 COMPLEXED WITH GDP, DIMERIC CRYSTAL FORM

Experimental procedure

Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1994-08-30
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	122.360, 45.010, 91.960
Unit cell angles	90.00, 133.62, 90.00

Refinement procedure

Resolution	8.000 - 2.400
R-factor	0.187
Rwork	0.187
R-free	0.31500
RMSD bond length	0.011
RMSD bond angle	2.010
Data reduction software	MOSFLM
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.400
Rmerge	0.081
Total number of observations	40351 *
Number of reflections	13030
Completeness [%]	90.0
Redundancy	3.01

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6 *		protein solution is mixed in a 1:1 ratio with well solution *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	reservoir	PEG8000	22 (%)
3	1	reservoir	ammonium sulfate	0.2 (M)
4	1	reservoir	MES	0.1 (M)