1RRA
RIBONUCLEASE A FROM RATTUS NORVEGICUS (COMMON RAT)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 1993-07 |
Detector | MARRESEARCH |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 69.130, 69.130, 60.930 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 8.000 - 2.500 |
R-factor | 0.186 * |
Rwork | 0.186 |
R-free | 0.27700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7rsa |
RMSD bond length | 0.012 |
RMSD bond angle | 26.180 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 8.000 | |
High resolution limit [Å] | 2.500 | 2.500 * |
Rmerge | 0.106 * | 0.156 * |
Number of reflections | 4534 | |
Completeness [%] | 77.0 | 60 * |
Redundancy | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 5.5 | 35% PEG-8K IN 10MM ACETATE BUFFER PH 5.5 CONTAINING 1MM DISODIUM HYDROGEN PHOSPHATE AND 10 MG/ML PROTEIN |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | drop | acetate | 10 (mM) | |
3 | 1 | drop | disodium hydrogen phosphate | 1 (mM) | |
4 | 1 | reservoir | PEG8000 | 35 (%) |