1ROZ
Deoxyhypusine synthase holoenzyme in its low ionic strength, high pH crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9B |
| Synchrotron site | NSLS |
| Beamline | X9B |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 1999-06-17 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 104.780, 104.780, 159.670 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.000 - 2.210 |
| Rwork | 0.178 |
| R-free | 0.19990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dhs |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.290 |
| High resolution limit [Å] | 2.210 | 2.210 |
| Number of reflections | 50963 | |
| <I/σ(I)> | 9.7 | |
| Completeness [%] | 99.9 | 99.3 |
| Redundancy | 3.85 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | Tris-HCl, KCl, NAD, 2-methyl-2,4-pentanediol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
