1RNM

RIBONUCLEASE A COMPLEX WITH CYTIDYLIC ACID (5'CMP) CRYSTALLIZED FROM 80% AMMONIUM SULPHATE

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	290
Detector technology	AREA DETECTOR
Collection date	1992
Detector	SIEMENS
Spacegroup name	P 32 2 1
Unit cell lengths	65.630, 65.630, 65.380
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 2.000
R-factor	0.158
Rwork	0.158
RMSD bond length	0.012
RMSD bond angle	2.470
Data reduction software	XDS
Data scaling software	XDS
Phasing software	X-PLOR
Refinement software	PROFFT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	9.600	2.090
High resolution limit [Å]	2.000	2.000
Rmerge	0.044	0.127
Number of reflections	8914
<I/σ(I)>	41.2	9.6
Completeness [%]	78.3	51.2
Redundancy	2.58	1.68

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.5		pH 5.5