1RL8
Crystal structure of the complex of resistant strain of hiv-1 protease(v82a mutant) with ritonavir
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-02-11 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.804 |
| Spacegroup name | P 61 |
| Unit cell lengths | 61.414, 61.414, 80.816 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.710 - 2.000 |
| R-factor | 0.226 |
| Rwork | 0.226 |
| R-free | 0.26900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nh0 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.900 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.710 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 11680 | |
| <I/σ(I)> | 28 | 4.5 |
| Completeness [%] | 99.5 | 96.3 |
| Redundancy | 20.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 300 | 50mM MES pH 6.5, 1.8M Ammonium Sulphate, 30% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
