1RJB
Crystal Structure of FLT3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-05-14 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 80.651, 80.651, 150.120 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.260 - 2.100 |
| R-factor | 0.22 |
| Rwork | 0.210 |
| R-free | 0.24600 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 * |
| RMSD bond angle | 1.590 * |
| Data reduction software | HKL-2000 |
| Data scaling software | CrystalClear ((MSC/RIGAKU)) |
| Phasing software | AMoRE |
| Refinement software | CNX (2000) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 * | 2.230 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.056 | 0.265 * |
| Number of reflections | 29690 * | |
| <I/σ(I)> | 18.6 | |
| Completeness [%] | 99.3 * | 94.1 |
| Redundancy | 6.82 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | sodium phosphate, potassium phosphate, CAPS, lithium sulfate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | sodium phosphate | 1.2 (M) | |
| 2 | 1 | reservoir | potassium phosphate | 1.2 (M) | |
| 3 | 1 | reservoir | CAPS | 1.2 (M) | |
| 4 | 1 | reservoir | lithium sulfate | 0.2 (M) | |
| 5 | 1 | drop | protein | 10 (mg/ml) | |
| 6 | 1 | drop | AMP-PNP | 2 (mM) | |
| 7 | 1 | drop | 4 (mM) |
