1RI4
Structure and mechanism of mRNA cap (guanine N-7) methyltransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 63.803, 63.803, 112.683 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 * - 2.400 |
| R-factor | 0.215 |
| Rwork | 0.215 |
| R-free | 0.28000 * |
| Structure solution method | SAD+MIR |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.150 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.066 | 0.231 * |
| Total number of observations | 124773 * | |
| Number of reflections | 9398 | |
| <I/σ(I)> | 26.4 | |
| Completeness [%] | 86.5 | 64.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8 * | 291 | 1.2M Na/K tartrate, 50mM BIS/TRIS, 20mM DTT, pH 6.25, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6.5 (mg/ml) | |
| 2 | 1 | drop | 220 (mM) | ||
| 3 | 1 | drop | Tris-HCl | 20 (mM) | pH8.0 |
| 4 | 1 | drop | beta-mercaptoethanol | 1 (mM) | |
| 5 | 1 | reservoir | sodium/pootassium tartrate | 1.2 (M) | |
| 6 | 1 | reservoir | bis-tris | 50 (mM) | pH6.0 |
| 7 | 1 | reservoir | dithiothreitol | 20 (mM) |
