1RF7
STRUCTURE OF DIHYDROFOLATE REDUCTASE COMPLEXED WITH DIHYDROFOLATE
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 277 |
Detector technology | AREA DETECTOR |
Collection date | 1995-11 |
Detector | XUONG-HAMLIN MULTIWIRE |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 34.705, 38.917, 108.827 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.800 |
R-factor | 0.17 * |
Rwork | 0.170 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1rx7 |
RMSD bond length | 0.018 |
RMSD bond angle | 3.010 |
Data reduction software | UCSD |
Data scaling software | UCSD |
Phasing software | MERLOT |
Refinement software | TNT (5D) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 1.890 |
High resolution limit [Å] | 1.800 | 1.750 |
Rmerge | 0.048 * | 0.143 * |
Total number of observations | 38377 * | |
Number of reflections | 14125 | |
<I/σ(I)> | 14.9 | 4.21 |
Completeness [%] | 94.0 | 76 |
Redundancy | 4.5 | 1.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | macro seeding * | 6.6 | pH 6.6 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | imidazole | 20 (mM) | |
2 | 1 | reservoir | PEG6000 | 16 (%(w/v)) | |
3 | 1 | reservoir | 210 (mM) | ||
4 | 1 | reservoir | KHP | 20 (mM) |