1R8H
Comparison of the structure and DNA binding properties of the E2 proteins from an oncogenic and a non-oncogenic human papillomavirus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-10-20 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.488 |
| Spacegroup name | P 61 |
| Unit cell lengths | 71.664, 71.664, 195.004 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.1947 |
| Rwork | 0.191 |
| R-free | 0.26280 * |
| Structure solution method | MIR |
| RMSD bond length | 0.019 * |
| RMSD bond angle | 1.691 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 * | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.044 * | 0.221 * |
| Number of reflections | 42743 | |
| Completeness [%] | 93.2 * | 58.8 |
| Redundancy | 3.0 * | 1.4 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | Ammonium sulfate, sodium chloride, sodium hepes, beta-mercaptoethanol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 2.5 (mg/ml) | |
| 2 | 1 | drop | beta-mercaptoethanol | 0.1 (%) | |
| 3 | 1 | drop | sodium azide | 0.02 (%(w/v)) | |
| 4 | 1 | reservoir | ammonium sulfate | 1-2 (M) | |
| 5 | 1 | reservoir | 0.1 (M) | ||
| 6 | 1 | reservoir | HEPES | 0.1 (M) | pH7.5 |
