1R7M
The homing endonuclease I-SceI bound to its DNA recognition region
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2001-03-19 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 80.600, 90.700, 115.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.350 - 2.250 |
| R-factor | 0.235 |
| Rwork | 0.235 |
| R-free | 0.26700 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.800 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Number of reflections | 36883 | |
| <I/σ(I)> | 18.1 | 18.1 |
| Completeness [%] | 91.7 | 91.7 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.7 | 277 | PEG400, sodium acetate, calcium chloride, postassium chloride, pH 4.7, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG400 | ||
| 2 | 1 | 1 | sodium acetate | ||
| 3 | 1 | 1 | calcium chloride | ||
| 4 | 1 | 1 | postassium chloride | ||
| 5 | 1 | 1 | H2O | ||
| 6 | 1 | 2 | PEG400 | ||
| 7 | 1 | 2 | calcium chloride | ||
| 8 | 1 | 2 | H2O |
