1R68
Role of the amino sugar in DNA binding of disaccharide anthracyclines: crystal structure of MAR70/d(CGATCG) complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 2003-02-15 |
| Detector | RIGAKU AFC-5R |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 28.190, 28.190, 53.250 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| Data reduction software | TEXAN |
| Data scaling software | TEXSAN |
| Phasing software | AMoRE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.300 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.114 | |
| Number of reflections | 6369 | |
| <I/σ(I)> | 4.3 | |
| Completeness [%] | 97.8 | 87 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 298 | Magnesium chloride, cacodylate, spermine, MPD, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K, pH 6.00 |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Magnesium chloride | ||
| 10 | 1 | 2 | H2O | ||
| 2 | 1 | 1 | cacodylate | ||
| 3 | 1 | 1 | spermine | ||
| 4 | 1 | 1 | MPD | ||
| 5 | 1 | 1 | H2O | ||
| 6 | 1 | 2 | Magnesium chloride | ||
| 7 | 1 | 2 | cacodylate | ||
| 8 | 1 | 2 | spermine | ||
| 9 | 1 | 2 | MPD |
