1R5U

RNA POLYMERASE II TFIIB COMPLEX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2003-01-23
Detector	ADSC QUANTUM 4
Spacegroup name	I 2 2 2
Unit cell lengths	212.438, 217.182, 422.876
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 4.500
R-factor	0.345
Rwork	0.345
R-free	0.37300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1i6h
RMSD bond length	0.024
RMSD bond angle	1.689
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	4.700
High resolution limit [Å]	4.500	4.500
Rmerge	0.091 *	0.277 *
Number of reflections	57906	8951 *
<I/σ(I)>	14	3.4
Completeness [%]	99.4	99.2
Redundancy	4.4	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	6.5 *	298	A.L., Gnatt, (2001) Science, 292, 1876. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	dioxane	50 (mM)
2	1	reservoir	dithiothreitol	10 (mM)
3	1	reservoir	ammonium phosphate	390 (mM)
4	1	reservoir	PEG6000