1R4I
Crystal Structure of Androgen Receptor DNA-Binding Domain Bound to a Direct Repeat Response Element
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-07-09 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0000, 1.2831 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 137.890, 137.890, 85.710 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 3.100 |
| Rwork | 0.246 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | ER Complex from PDB entry 1HCQ |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.096 | 0.620 |
| Number of reflections | 17313 | |
| <I/σ(I)> | 20.4 | 2.5 |
| Completeness [%] | 99.7 | 99.1 |
| Redundancy | 10.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | PEG 400, magnesium chloride, Tris, DTT, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 400 | ||
| 2 | 1 | 1 | magnesium chloride | ||
| 3 | 1 | 1 | Tris | ||
| 4 | 1 | 1 | DTT | ||
| 5 | 1 | 1 | H2O | ||
| 6 | 1 | 2 | PEG 400 | ||
| 7 | 1 | 2 | magnesium chloride | ||
| 8 | 1 | 2 | DTT | ||
| 9 | 1 | 2 | H2O |
