1QZF
Crystal structure of DHFR-TS from Cryptosporidium hominis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-04-05 |
| Detector | BRANDEIS - B4 |
| Wavelength(s) | 1.08 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 214.900, 116.300, 219.700 |
| Unit cell angles | 90.00, 95.23, 90.00 |
Refinement procedure
| Resolution | 29.800 - 2.800 |
| R-factor | 0.225 |
| Rwork | 0.225 |
| R-free | 0.24500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1f28 AND 1cd2 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.604 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.105 | |
| Number of reflections | 134842 | |
| <I/σ(I)> | 8.7 | |
| Completeness [%] | 87.9 | 52.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | PEG 6000, ammonium sulfate, lithium sulfate, Tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7 (mg/ml) | |
| 2 | 1 | reservoir | PEG6000 | 10 (%) | |
| 3 | 1 | reservoir | ammonium sulfate | 50 (mM) | |
| 4 | 1 | reservoir | lithium sulfate | 150 (mM) | |
| 5 | 1 | reservoir | Tris | 100 (mM) | pH8.0 |
