1QZ7
Beta-catenin binding domain of Axin in complex with beta-catenin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2002-12-05 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 85.141, 75.041, 101.398 |
| Unit cell angles | 90.00, 97.47, 90.00 |
Refinement procedure
| Resolution | 42.210 - 2.200 |
| R-factor | 0.221 |
| Rwork | 0.221 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1i7x |
| RMSD bond length | 0.006 |
| RMSD bond angle | 18.400 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.200 * | 2.100 |
| Rmerge | 0.072 | 0.529 * |
| Number of reflections | 37253 | |
| <I/σ(I)> | 17.8 | 1.8 |
| Completeness [%] | 99.8 | 99.9 * |
| Redundancy | 3.7 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 * | 20 * | PEG 6000, sodium citrate, isopropanol, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 2 (mg/ml) | |
| 2 | 1 | drop | Tris | 20 (mM) | pH8.5 |
| 3 | 1 | drop | 0.15 (M) | ||
| 4 | 1 | drop | dithiothreitol | 2 (mM) | |
| 5 | 1 | reservoir | sodium citrate | 0.1 (M) | pH5.6 |
| 6 | 1 | reservoir | isopropanol | 15 (%(v/v)) | |
| 7 | 1 | reservoir | PEG4000-6000 | 7 (%(w/v)) | |
| 8 | 1 | reservoir | dithiothreitol | 5 (mM) |
