1QX4
Structrue of S127P mutant of cytochrome b5 reductase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12B |
| Synchrotron site | NSLS |
| Beamline | X12B |
| Temperature [K] | 99 |
| Detector technology | CCD |
| Collection date | 2001-05-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 77.236, 48.004, 77.247 |
| Unit cell angles | 90.00, 107.69, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.800 |
| R-factor | 0.207 |
| Rwork | 0.207 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1IB0 without FAD molecule |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 1.800 |
| Rmerge | 0.064 |
| Number of reflections | 48325 |
| Completeness [%] | 96.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | glycerol, ammonium sulfate, tris buffer, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
