1QVY
Crystal structure of RhoGDI K(199,200)R double mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2002-07-03 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.9190 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 148.660, 58.240, 75.050 |
| Unit cell angles | 90.00, 92.49, 90.00 |
Refinement procedure
| Resolution | 27.020 - 1.600 |
| R-factor | 0.17281 |
| Rwork | 0.172 |
| R-free | 0.20998 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kmt |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.719 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.053 | 0.382 |
| Number of reflections | 84662 | |
| <I/σ(I)> | 15.2 | 2.7 |
| Completeness [%] | 99.6 | 99.4 |
| Redundancy | 3.7 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 294 | 30% PEG 400, 0.1 M Tris, 0.2 M lithium sulfate, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 294.0K |
