1QS0
Crystal Structure of Pseudomonas Putida 2-oxoisovalerate Dehydrogenase (Branched-Chain Alpha-Keto Acid Dehydrogenase, E1B)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-06-21 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.88561,0.97888,0.97894 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 101.340, 101.340, 381.230 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.400 |
| R-factor | 0.218 |
| Rwork | 0.218 |
| R-free | 0.26500 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | SHARP |
| Refinement software | CNS (0.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.000 | |
| High resolution limit [Å] | 2.400 | |
| Rmerge | 0.077 | 0.182 * |
| Total number of observations | 407418 * | |
| Number of reflections | 56728 * | |
| <I/σ(I)> | 16 | |
| Completeness [%] | 97.3 | 74 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 * | 295 | POTASSIUM PHOSPHATE, DTT, MAGNESIUM CHLORIDE, pH 5.2, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8 (mg/ml) | |
| 2 | 1 | drop | potassium phosphate | 50 (mM) | |
| 3 | 1 | drop | ThDP | 1 (mM) | |
| 4 | 1 | drop | 4 (mM) | ||
| 5 | 1 | drop | L-valine | 10 (mM) | |
| 6 | 1 | drop | dithiothreitol | 4-12 (mM) | |
| 7 | 1 | reservoir | sodium potassium phosphate | 1.8-2.5 (M) | |
| 8 | 1 | reservoir | 0.01 (%) | ||
| 9 | 1 | reservoir | dithiothreitol | 4-12 (mM) |
