1QQT
METHIONYL-TRNA SYNTHETASE FROM ESCHERICHIA COLI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE DW32 |
| Synchrotron site | LURE |
| Beamline | DW32 |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-07-05 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 78.150, 46.300, 87.700 |
| Unit cell angles | 90.00, 109.06, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.030 |
| R-factor | 0.174 |
| Rwork | 0.170 |
| R-free | 0.21400 |
| Structure solution method | MIR and molecular replacement |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.100 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((AGROVATA) |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.080 |
| High resolution limit [Å] | 2.030 | 2.030 |
| Rmerge | 0.053 * | 0.129 |
| Number of reflections | 38431 | |
| <I/σ(I)> | 10.7 | |
| Completeness [%] | 98.4 | 80 |
| Redundancy | 3.2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7 | 277 | 1.08M AMMONIUM CITRATE, 30MM POTASSIUM PHOSPHATE, pH 7.0, VAPOR DIFFUSION, HANGING DROP at 277K, temperature 277.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | enzyme | 3.6 (mg/ml) | |
| 2 | 1 | drop | ammonium citrate | 1.08 (M) | |
| 3 | 1 | drop | potassium phosphate | 30 (mM) | |
| 4 | 1 | drop | 2-mercaptoethanol | 4 (mM) |
