1QPG

3-PHOSPHOGLYCERATE KINASE, MUTATION R65Q

Experimental procedure

Detector technology	AREA DETECTOR
Collection date	1991-09-30
Detector	SIEMENS
Spacegroup name	C 1 2 1
Unit cell lengths	96.000, 70.100, 82.300
Unit cell angles	90.00, 122.20, 90.00

Refinement procedure

Resolution	8.000 - 2.400
R-factor	0.185
Rwork	0.185
RMSD bond length	0.009
RMSD bond angle	21.670 *
Data reduction software	XENGEN
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	40.700
High resolution limit [Å]	2.430
Rmerge	0.076
Total number of observations	100828 *
Number of reflections	16281
Completeness [%]	92.4
Redundancy	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	R65Q PGK	18 (mg/ml)
2	1	drop	5'-adenylylimidodiphosphate	10 (mM)
3	1	drop	tetralithium salt (AMP-PNP)
4	1	drop	D-(-)-3-phosphoglycerate	10 (mM)
5	1	drop	disodium salt (3-PG)
6	1	reservoir	PEG8000	21 (%)
7	1	reservoir	magnesium acetate	20 (mM)
8	1	reservoir		0.02 (%)
9	1	reservoir	HEPES	50 (mM)