1QO8
The structure of the open conformation of a flavocytochrome c3 fumarate reductase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-04-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8469, 1.033, 1.739, 1.741 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 71.770, 109.690, 227.320 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.150 |
| R-factor | 0.225 * |
| Rwork | 0.225 |
| R-free | 0.28100 |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 0.013 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.190 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.038 * | 0.274 * |
| Total number of observations | 1428311 * | |
| Number of reflections | 97968 | |
| Completeness [%] | 93.4 * | 91.4 |
| Redundancy | 14.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | Bamford, V., (1999) Acta Crystallogr.,Sect., D55, 1222. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10-15 (mg/ml) | |
| 2 | 1 | drop | sodium HEPES | 50 (mM) | |
| 3 | 1 | drop | 100 (mM) | ||
| 4 | 1 | reservoir | PEG10000 | 6-15 (%(w/v)) | |
| 5 | 1 | reservoir | sodium MES |
