1QE6

INTERLEUKIN-8 WITH AN ADDED DISULFIDE BETWEEN RESIDUES 5 AND 33 (L5C/H33C)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	1996-10-15
Detector	PRINCETON 1K
Spacegroup name	P 1 21 1
Unit cell lengths	37.540, 71.770, 57.860
Unit cell angles	90.00, 90.46, 90.00

Refinement procedure

Resolution	20.000 - 2.350
R-factor	0.206 *
Rwork	0.208
R-free	0.27700 *
RMSD bond length	0.006 *
RMSD bond angle	1.000
Data reduction software	MCCDATA
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.430
High resolution limit [Å]	2.350	2.350
Rmerge	0.088	0.113
Number of reflections	12188
<I/σ(I)>	13
Completeness [%]	95.0	86
Redundancy	4.3	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	20 *	NACL, AMMONIUM SULFATE, PEG 8000, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 19K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	4.2 (mg/ml)
2	1	drop		100 (mM)
3	1	drop	MES	4 (mM)
4	1	drop	ammonium sulfate	60 (mM)
5	1	drop	PEG8000	18 (%(w/v))
6	1	reservoir	ammonium sulfate	100 (mM)
7	1	reservoir	PEG8000	30 (%(w/v))