1QE1
CRYSTAL STRUCTURE OF 3TC-RESISTANT M184I MUTANT OF HIV-1 REVERSE TRANSCRIPTASE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 108 |
Detector technology | CCD |
Detector | PRINCETON 2K |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 238.600, 72.700, 95.300 |
Unit cell angles | 90.00, 105.60, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.850 |
R-factor | 0.259 * |
Rwork | 0.259 |
R-free | 0.34600 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.690 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | X-PLOR (3.843) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.950 |
High resolution limit [Å] | 2.850 | 2.850 |
Rmerge | 0.090 | 0.360 |
Number of reflections | 35177 | |
<I/σ(I)> | 5.2 | |
Completeness [%] | 95.0 | 88 |
Redundancy | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 277 | PEG 8000, GLYCEROL, BIS-TRIS, AMMONIUM SULFATE, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | bis-Tris | 50 (mM) | |
2 | 1 | reservoir | ammonium sulfate | 100 (mM) | |
3 | 1 | reservoir | glycerol | 10 (%) | |
4 | 1 | reservoir | PEG8000 | 9 (%) |