1QC1
CRYSTAL STRUCTURE OF THE SELF-FITTED B-DNA DECAMER D(CCGCCGGCGG)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 258 |
| Detector technology | AREA DETECTOR |
| Detector | SIEMENS |
| Spacegroup name | H 3 |
| Unit cell lengths | 53.700, 53.700, 45.000 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 7.000 - 2.500 |
| R-factor | 0.147 |
| Data reduction software | X-GEN |
| Data scaling software | X-GEN |
| Refinement software | NUCLSQ |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.400 |
| Rmerge | 0.078 |
| Total number of observations | 6367 * |
| Number of reflections | 1817 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6 | 4 * | 30 % MPD, 1MM DNA DUPLEX, 1 MM SPERMINE, 20 MM MAGNESIUM ACETATE, 50 MM CACODYLATE BUFFER, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 2 | 1 | 1 | SPERMINE | ||
| 3 | 1 | 1 | MAGNESIUM ACETATE | ||
| 4 | 1 | 1 | CACODYLATE BUFFER | ||
| 5 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | cacodylate | 50 (mM) | |
| 2 | 1 | drop | dodecamer duplex | 1 (mM) | |
| 3 | 1 | drop | spermine tetrahydrochloride | 1.2 (mM) | |
| 4 | 1 | drop | magnesium acetate | 18 (mM) | |
| 5 | 1 | reservoir | 45 (%) |
