1QAS

1-PHOSPHATIDYLINOSITOL-4,5-BISPHOSPHATE PHOSPHODIESTERASE DELTA 1

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1995-10
Detector	RIGAKU
Spacegroup name	P 1
Unit cell lengths	61.100, 75.400, 86.900
Unit cell angles	66.90, 85.40, 89.80

Refinement procedure

Resolution	6.000 - 2.400
R-factor	0.212
Rwork	0.212
R-free	0.28600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	CUBIC PHOSPHOLIPASE C
RMSD bond length	0.011
RMSD bond angle	24.900 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.063	0.296
Number of reflections	47893
<I/σ(I)>	10.9	2.1
Completeness [%]	86.4	44.5
Redundancy	1.76	1.42

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5		used to seeding, Grobler, J.A., (1996) Protein Sci., 5, 680. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	NaMes	0.1 (M)
2	1	reservoir		0.2 (M)
3	1	reservoir	glycerol	20 (%)
4	1	reservoir	PEG8000	6-8 (%)