1Q7L
Zn-binding domain of the T347G mutant of human aminoacylase-I
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-04-20 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.005685 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.528, 67.249, 146.479 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 * - 1.400 |
| R-factor | 0.13299 |
| Rwork | 0.133 |
| R-free | 0.17200 * |
| Structure solution method | SIRAS |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.890 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.08) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 * | |
| High resolution limit [Å] | 1.500 * | 1.500 * |
| Rmerge | 0.074 * | 0.344 * |
| Total number of observations | 529534 * | |
| Number of reflections | 158345 * | |
| Completeness [%] | 95.8 * | 81.9 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 293 | PEG8000, (NH4)2SO4, CoCl2, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5-8 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 20 (mM) | pH7.5 |
| 3 | 1 | reservoir | PEG8000 | 20 (%(w/v)) | |
| 4 | 1 | reservoir | ammonium sulfate | 0.2 (M) | |
| 5 | 1 | reservoir | 2 (mM) |
