1Q5M

Binary complex of rabbit 20alpha-hydroxysteroid dehydrogenase with NADPH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	200
Detector technology	CCD
Collection date	2001-04-27
Detector	ADSC QUANTUM 4
Wavelength(s)	0.933
Spacegroup name	P 1 21 1
Unit cell lengths	57.540, 85.270, 66.150
Unit cell angles	90.00, 91.48, 90.00

Refinement procedure

Resolution	57.740 - 1.320
R-factor	0.14918
Rwork	0.148
R-free	0.17690
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.033
RMSD bond angle	2.607
Data scaling software	XDS
Phasing software	AMoRE
Refinement software	REFMAC (5.1.9999)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.740	1.370
High resolution limit [Å]	1.320	1.320
Rmerge	0.069
Number of reflections	141980
<I/σ(I)>	15.1
Completeness [%]		69.1
Redundancy	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	298	LiSO4 and PEG 4K, pH 7.5, VAPOR DIFFUSION