1Q3U
Crystal structure of a wild-type Cre recombinase-loxP synapse: pre-cleavage complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 107.991, 161.064, 196.003 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.030 - 2.800 * |
| R-factor | 0.223 |
| Rwork | 0.223 |
| R-free | 0.25200 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1crx |
| RMSD bond length | 0.007 |
| RMSD bond angle | 19.900 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.073 * | 0.318 * |
| Completeness [%] | 97.4 | 94.6 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7.6 | 293 | NaCl, EDTA, Glycerol, HEPES, MgCl2, PEG 2000, MME, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MME | ||
| 10 | 1 | 2 | Glycerol | ||
| 11 | 1 | 2 | PEG 2000 | ||
| 2 | 1 | 1 | EDTA | ||
| 3 | 1 | 1 | HEPES | ||
| 4 | 1 | 1 | NaCl | ||
| 5 | 1 | 1 | MgCl2 | ||
| 6 | 1 | 1 | Glycerol | ||
| 7 | 1 | 1 | PEG 2000 | ||
| 8 | 1 | 2 | NaCl | ||
| 9 | 1 | 2 | MgCl2 |
