1Q32
Crystal Structure Analysis of the Yeast Tyrosyl-DNA Phosphodiesterase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12B |
| Synchrotron site | NSLS |
| Beamline | X12B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-10-24 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9789, 0.9796, 0.9637 |
| Spacegroup name | P 1 |
| Unit cell lengths | 64.163, 82.207, 98.442 |
| Unit cell angles | 89.39, 85.81, 67.12 |
Refinement procedure
| Resolution | 30.000 - 2.030 |
| R-factor | 0.21411 |
| Rwork | 0.214 |
| R-free | 0.24140 |
| Structure solution method | MAD |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.067 | |
| Number of reflections | 155815 | |
| Completeness [%] | 90.4 | 54.3 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 7.8 | 291.15 | PEG 4000, Hepes, LiSO4, DTT, 1.6.Hexanediol , pH 7.8, EVAPORATION, temperature 291.15K |
| 1 | EVAPORATION | 7.8 | 291.15 | PEG 4000, Hepes, LiSO4, DTT, 1.6.Hexanediol , pH 7.8, EVAPORATION, temperature 291.15K |
